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human sclc cell lines 21h  (Procell Inc)

 
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    Procell Inc human sclc cell lines 21h
    Fibroblast-derived IL-6 promoted phenotypic reprogramming of <t>SCLC</t> cells. ( a , b <t>)</t> <t>H69</t> and <t>H2227</t> cells co-cultured with MRC-5 cells ( a ) or HFL1 ( b ) respectively and the expression of NE genes in H69 and H2227 cells was measured by qRT-PCR (n = 3); ( c ) The protein expression of NE markers was determined by Western blot analysis and the blots were cropped prior to hybridisation with antibodies, original images were displayed in . ( d , e ) Antibody arrays were performed in serum-free culture medium from separately cultured or co-cultured H2227 and MRC-5 cells, membranes were scanned by chemiluminescence instrument ( d ) and quantified by image J software, the levels of cytokines were normalized to positive controls ( e ); ( f ) The expression of NE genes in H2227 cells cultured separately or co-cultured with MRC-5 cells which transfected with siRNA targeted to IL6, CCL20 or IGFBP1 or negative control were determined by qRT-PCR (n = 3); ( g ) Western blot was used to analyze the expression of NE markers in H69 and H2227 cells which cultured separately or co-cultured with MRC-5 cells which transfected with siRNA targeted to IL6 or negative control, the blots were cropped prior to hybridisation with antibodies, original images were displayed in . Quantification of blots were performed using Image J software (n = 2). * P < 0.05, ** P < 0.01.
    Human Sclc Cell Lines 21h, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human sclc cell lines 21h/product/Procell Inc
    Average 90 stars, based on 1 article reviews
    human sclc cell lines 21h - by Bioz Stars, 2026-03
    90/100 stars

    Images

    1) Product Images from "Dynamic phenotypic reprogramming and chemoresistance induced by lung fibroblasts in small cell lung cancer"

    Article Title: Dynamic phenotypic reprogramming and chemoresistance induced by lung fibroblasts in small cell lung cancer

    Journal: Scientific Reports

    doi: 10.1038/s41598-024-52687-z

    Fibroblast-derived IL-6 promoted phenotypic reprogramming of SCLC cells. ( a , b ) H69 and H2227 cells co-cultured with MRC-5 cells ( a ) or HFL1 ( b ) respectively and the expression of NE genes in H69 and H2227 cells was measured by qRT-PCR (n = 3); ( c ) The protein expression of NE markers was determined by Western blot analysis and the blots were cropped prior to hybridisation with antibodies, original images were displayed in . ( d , e ) Antibody arrays were performed in serum-free culture medium from separately cultured or co-cultured H2227 and MRC-5 cells, membranes were scanned by chemiluminescence instrument ( d ) and quantified by image J software, the levels of cytokines were normalized to positive controls ( e ); ( f ) The expression of NE genes in H2227 cells cultured separately or co-cultured with MRC-5 cells which transfected with siRNA targeted to IL6, CCL20 or IGFBP1 or negative control were determined by qRT-PCR (n = 3); ( g ) Western blot was used to analyze the expression of NE markers in H69 and H2227 cells which cultured separately or co-cultured with MRC-5 cells which transfected with siRNA targeted to IL6 or negative control, the blots were cropped prior to hybridisation with antibodies, original images were displayed in . Quantification of blots were performed using Image J software (n = 2). * P < 0.05, ** P < 0.01.
    Figure Legend Snippet: Fibroblast-derived IL-6 promoted phenotypic reprogramming of SCLC cells. ( a , b ) H69 and H2227 cells co-cultured with MRC-5 cells ( a ) or HFL1 ( b ) respectively and the expression of NE genes in H69 and H2227 cells was measured by qRT-PCR (n = 3); ( c ) The protein expression of NE markers was determined by Western blot analysis and the blots were cropped prior to hybridisation with antibodies, original images were displayed in . ( d , e ) Antibody arrays were performed in serum-free culture medium from separately cultured or co-cultured H2227 and MRC-5 cells, membranes were scanned by chemiluminescence instrument ( d ) and quantified by image J software, the levels of cytokines were normalized to positive controls ( e ); ( f ) The expression of NE genes in H2227 cells cultured separately or co-cultured with MRC-5 cells which transfected with siRNA targeted to IL6, CCL20 or IGFBP1 or negative control were determined by qRT-PCR (n = 3); ( g ) Western blot was used to analyze the expression of NE markers in H69 and H2227 cells which cultured separately or co-cultured with MRC-5 cells which transfected with siRNA targeted to IL6 or negative control, the blots were cropped prior to hybridisation with antibodies, original images were displayed in . Quantification of blots were performed using Image J software (n = 2). * P < 0.05, ** P < 0.01.

    Techniques Used: Derivative Assay, Cell Culture, Expressing, Quantitative RT-PCR, Western Blot, Hybridization, Software, Transfection, Negative Control

    Evaluation and validation of drug sensitivity upon fibroblasts stimulation in SCLC. ( a , b ) The log fold change of predicted sensitivity scores of drugs based on GDSC2 database between high- and low-infiltration group in George’s cohort, Log FC > 0 means high-infiltration group is more resistant to these drugs (blue bars), Log FC < 0 represents high-infiltration group is more sensitive to these drugs (orange bars); ( c , d ) NE type SCLC cells were separately cultured or co-cultured with MRC-5 for 7 days, then reseeded and treated with cisplatin or etoposide for another 48 h, cell viability was detected by CCK-8 reagent (n = 3). ( e , f ) CCK-8 reagent was used to test the viability of separately cultured or co-cultured H69 and H2227 cells which pretreated with or without AZD-1480 and exposed to Cisplatin ( e ) or Etoposide ( f ). * or #, P < 0.05, ** or ##, P < 0.01. *, Co-Veh vs. Mo-Veh. #, Co-AZD vs. Mo-AZD.
    Figure Legend Snippet: Evaluation and validation of drug sensitivity upon fibroblasts stimulation in SCLC. ( a , b ) The log fold change of predicted sensitivity scores of drugs based on GDSC2 database between high- and low-infiltration group in George’s cohort, Log FC > 0 means high-infiltration group is more resistant to these drugs (blue bars), Log FC < 0 represents high-infiltration group is more sensitive to these drugs (orange bars); ( c , d ) NE type SCLC cells were separately cultured or co-cultured with MRC-5 for 7 days, then reseeded and treated with cisplatin or etoposide for another 48 h, cell viability was detected by CCK-8 reagent (n = 3). ( e , f ) CCK-8 reagent was used to test the viability of separately cultured or co-cultured H69 and H2227 cells which pretreated with or without AZD-1480 and exposed to Cisplatin ( e ) or Etoposide ( f ). * or #, P < 0.05, ** or ##, P < 0.01. *, Co-Veh vs. Mo-Veh. #, Co-AZD vs. Mo-AZD.

    Techniques Used: Biomarker Discovery, Cell Culture, CCK-8 Assay



    Similar Products

    human sclc cell lines 21h  (Procell Inc)
    90
    Procell Inc human sclc cell lines 21h
    Fibroblast-derived IL-6 promoted phenotypic reprogramming of <t>SCLC</t> cells. ( a , b <t>)</t> <t>H69</t> and <t>H2227</t> cells co-cultured with MRC-5 cells ( a ) or HFL1 ( b ) respectively and the expression of NE genes in H69 and H2227 cells was measured by qRT-PCR (n = 3); ( c ) The protein expression of NE markers was determined by Western blot analysis and the blots were cropped prior to hybridisation with antibodies, original images were displayed in . ( d , e ) Antibody arrays were performed in serum-free culture medium from separately cultured or co-cultured H2227 and MRC-5 cells, membranes were scanned by chemiluminescence instrument ( d ) and quantified by image J software, the levels of cytokines were normalized to positive controls ( e ); ( f ) The expression of NE genes in H2227 cells cultured separately or co-cultured with MRC-5 cells which transfected with siRNA targeted to IL6, CCL20 or IGFBP1 or negative control were determined by qRT-PCR (n = 3); ( g ) Western blot was used to analyze the expression of NE markers in H69 and H2227 cells which cultured separately or co-cultured with MRC-5 cells which transfected with siRNA targeted to IL6 or negative control, the blots were cropped prior to hybridisation with antibodies, original images were displayed in . Quantification of blots were performed using Image J software (n = 2). * P < 0.05, ** P < 0.01.
    Human Sclc Cell Lines 21h, supplied by Procell Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human sclc cell lines 21h/product/Procell Inc
    Average 90 stars, based on 1 article reviews
    human sclc cell lines 21h - by Bioz Stars, 2026-03
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Fibroblast-derived IL-6 promoted phenotypic reprogramming of SCLC cells. ( a , b ) H69 and H2227 cells co-cultured with MRC-5 cells ( a ) or HFL1 ( b ) respectively and the expression of NE genes in H69 and H2227 cells was measured by qRT-PCR (n = 3); ( c ) The protein expression of NE markers was determined by Western blot analysis and the blots were cropped prior to hybridisation with antibodies, original images were displayed in . ( d , e ) Antibody arrays were performed in serum-free culture medium from separately cultured or co-cultured H2227 and MRC-5 cells, membranes were scanned by chemiluminescence instrument ( d ) and quantified by image J software, the levels of cytokines were normalized to positive controls ( e ); ( f ) The expression of NE genes in H2227 cells cultured separately or co-cultured with MRC-5 cells which transfected with siRNA targeted to IL6, CCL20 or IGFBP1 or negative control were determined by qRT-PCR (n = 3); ( g ) Western blot was used to analyze the expression of NE markers in H69 and H2227 cells which cultured separately or co-cultured with MRC-5 cells which transfected with siRNA targeted to IL6 or negative control, the blots were cropped prior to hybridisation with antibodies, original images were displayed in . Quantification of blots were performed using Image J software (n = 2). * P < 0.05, ** P < 0.01.

    Journal: Scientific Reports

    Article Title: Dynamic phenotypic reprogramming and chemoresistance induced by lung fibroblasts in small cell lung cancer

    doi: 10.1038/s41598-024-52687-z

    Figure Lengend Snippet: Fibroblast-derived IL-6 promoted phenotypic reprogramming of SCLC cells. ( a , b ) H69 and H2227 cells co-cultured with MRC-5 cells ( a ) or HFL1 ( b ) respectively and the expression of NE genes in H69 and H2227 cells was measured by qRT-PCR (n = 3); ( c ) The protein expression of NE markers was determined by Western blot analysis and the blots were cropped prior to hybridisation with antibodies, original images were displayed in . ( d , e ) Antibody arrays were performed in serum-free culture medium from separately cultured or co-cultured H2227 and MRC-5 cells, membranes were scanned by chemiluminescence instrument ( d ) and quantified by image J software, the levels of cytokines were normalized to positive controls ( e ); ( f ) The expression of NE genes in H2227 cells cultured separately or co-cultured with MRC-5 cells which transfected with siRNA targeted to IL6, CCL20 or IGFBP1 or negative control were determined by qRT-PCR (n = 3); ( g ) Western blot was used to analyze the expression of NE markers in H69 and H2227 cells which cultured separately or co-cultured with MRC-5 cells which transfected with siRNA targeted to IL6 or negative control, the blots were cropped prior to hybridisation with antibodies, original images were displayed in . Quantification of blots were performed using Image J software (n = 2). * P < 0.05, ** P < 0.01.

    Article Snippet: Human SCLC cell lines (H69, H2227, H1092, H446, 21H, H196 and SBC-5) were obtained from National collection of Authenticated cell cultures (Shanghai, China) or Procell Life Science and Technology company (Wuhan, Hubei, China) in 2021.

    Techniques: Derivative Assay, Cell Culture, Expressing, Quantitative RT-PCR, Western Blot, Hybridization, Software, Transfection, Negative Control

    Evaluation and validation of drug sensitivity upon fibroblasts stimulation in SCLC. ( a , b ) The log fold change of predicted sensitivity scores of drugs based on GDSC2 database between high- and low-infiltration group in George’s cohort, Log FC > 0 means high-infiltration group is more resistant to these drugs (blue bars), Log FC < 0 represents high-infiltration group is more sensitive to these drugs (orange bars); ( c , d ) NE type SCLC cells were separately cultured or co-cultured with MRC-5 for 7 days, then reseeded and treated with cisplatin or etoposide for another 48 h, cell viability was detected by CCK-8 reagent (n = 3). ( e , f ) CCK-8 reagent was used to test the viability of separately cultured or co-cultured H69 and H2227 cells which pretreated with or without AZD-1480 and exposed to Cisplatin ( e ) or Etoposide ( f ). * or #, P < 0.05, ** or ##, P < 0.01. *, Co-Veh vs. Mo-Veh. #, Co-AZD vs. Mo-AZD.

    Journal: Scientific Reports

    Article Title: Dynamic phenotypic reprogramming and chemoresistance induced by lung fibroblasts in small cell lung cancer

    doi: 10.1038/s41598-024-52687-z

    Figure Lengend Snippet: Evaluation and validation of drug sensitivity upon fibroblasts stimulation in SCLC. ( a , b ) The log fold change of predicted sensitivity scores of drugs based on GDSC2 database between high- and low-infiltration group in George’s cohort, Log FC > 0 means high-infiltration group is more resistant to these drugs (blue bars), Log FC < 0 represents high-infiltration group is more sensitive to these drugs (orange bars); ( c , d ) NE type SCLC cells were separately cultured or co-cultured with MRC-5 for 7 days, then reseeded and treated with cisplatin or etoposide for another 48 h, cell viability was detected by CCK-8 reagent (n = 3). ( e , f ) CCK-8 reagent was used to test the viability of separately cultured or co-cultured H69 and H2227 cells which pretreated with or without AZD-1480 and exposed to Cisplatin ( e ) or Etoposide ( f ). * or #, P < 0.05, ** or ##, P < 0.01. *, Co-Veh vs. Mo-Veh. #, Co-AZD vs. Mo-AZD.

    Article Snippet: Human SCLC cell lines (H69, H2227, H1092, H446, 21H, H196 and SBC-5) were obtained from National collection of Authenticated cell cultures (Shanghai, China) or Procell Life Science and Technology company (Wuhan, Hubei, China) in 2021.

    Techniques: Biomarker Discovery, Cell Culture, CCK-8 Assay